Eight employees from Ford Farm at Litton Cheney entered the event at Kingston Maurward and raised over £2,000 for Cancer Research UK.
The team completed the course dressed as a herd of dairy cows, with Paulina Dziechciarz first back in just 29 minutes.
The women were also joined by team member Debbie Wallis’ two Labradors, Maggie and Lottie who donned cow print tutus.
After three months of fundraising the team quadrupled their original target of £500, raising £2,099.50 thanks to generous donations from friends, family and customers including Omsco, Atlas Packaging, Berkshire Labels and Godminster.
Team leader Sian Whitlock said: “It was a really hot day so it was quite a challenge running in the costumes but well worth the effort. “There were hundreds of people taking part in the race.
“We are staggered by the generosity of all of our sponsors and really hope that the money we have managed to raise will help make a difference to those affected by cancer.”